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fibronectin  (Thermo Fisher)


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    Structured Review

    Thermo Fisher fibronectin
    Fibronectin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fibronectin+cs1+peptide/Fibronectin+CS1+Peptide/bio_rxiv__64898__2026__03__19__712761-240-11-12
    Average 94 stars, based on 1 article reviews
    fibronectin - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Oncofetal Chondroitin Sulfate Glycosaminoglycans are Key Players in Integrin Signaling and Tumor Cell Motility
    Article Snippet: cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs. .. The cells were stimulated with 1-3% FBS, 5ug/ml FN (Sigma) or 10-80ug/ml fibronectin CS1 peptide (GeneArt) for the given timepoints. .. The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Pho

    Article Title: Oncofetal Chondroitin Sulfate Glycosaminoglycans are Key Players in Integrin Signaling and Tumor Cell Motility
    Article Snippet: The cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs.The cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs.. The cells were stimulated with 1-3% FBS, 5ug/ml FN (Sigma) or 10-80ug/ml fibronectin CS1 peptide (GeneArt) for the given timepoints.. The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Phosphatase and protease inhibitor cocktails (Roche).The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Phosphatase and protease inhibitor cocktails (Roche).

    Phospho-proteomics:

    Article Title: Oncofetal Chondroitin Sulfate Glycosaminoglycans are Key Players in Integrin Signaling and Tumor Cell Motility
    Article Snippet: cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs. .. The cells were stimulated with 1-3% FBS, 5ug/ml FN (Sigma) or 10-80ug/ml fibronectin CS1 peptide (GeneArt) for the given timepoints. .. The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Pho

    Article Title: Oncofetal Chondroitin Sulfate Glycosaminoglycans are Key Players in Integrin Signaling and Tumor Cell Motility
    Article Snippet: The cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs.The cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs.. The cells were stimulated with 1-3% FBS, 5ug/ml FN (Sigma) or 10-80ug/ml fibronectin CS1 peptide (GeneArt) for the given timepoints.. The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Phosphatase and protease inhibitor cocktails (Roche).The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Phosphatase and protease inhibitor cocktails (Roche).

    Western Blot:

    Article Title: Oncofetal Chondroitin Sulfate Glycosaminoglycans are Key Players in Integrin Signaling and Tumor Cell Motility
    Article Snippet: cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs. .. The cells were stimulated with 1-3% FBS, 5ug/ml FN (Sigma) or 10-80ug/ml fibronectin CS1 peptide (GeneArt) for the given timepoints. .. The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Pho

    Article Title: Oncofetal Chondroitin Sulfate Glycosaminoglycans are Key Players in Integrin Signaling and Tumor Cell Motility
    Article Snippet: The cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs.The cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs.. The cells were stimulated with 1-3% FBS, 5ug/ml FN (Sigma) or 10-80ug/ml fibronectin CS1 peptide (GeneArt) for the given timepoints.. The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Phosphatase and protease inhibitor cocktails (Roche).The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Phosphatase and protease inhibitor cocktails (Roche).

    Titration:

    Article Title: Oncofetal Chondroitin Sulfate Glycosaminoglycans are Key Players in Integrin Signaling and Tumor Cell Motility
    Article Snippet: cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs. .. The cells were stimulated with 1-3% FBS, 5ug/ml FN (Sigma) or 10-80ug/ml fibronectin CS1 peptide (GeneArt) for the given timepoints. .. The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Pho

    Article Title: Oncofetal Chondroitin Sulfate Glycosaminoglycans are Key Players in Integrin Signaling and Tumor Cell Motility
    Article Snippet: The cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs.The cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs.. The cells were stimulated with 1-3% FBS, 5ug/ml FN (Sigma) or 10-80ug/ml fibronectin CS1 peptide (GeneArt) for the given timepoints.. The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Phosphatase and protease inhibitor cocktails (Roche).The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Phosphatase and protease inhibitor cocktails (Roche).

    Flow Cytometry:

    Article Title: Oncofetal Chondroitin Sulfate Glycosaminoglycans are Key Players in Integrin Signaling and Tumor Cell Motility
    Article Snippet: cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs. .. The cells were stimulated with 1-3% FBS, 5ug/ml FN (Sigma) or 10-80ug/ml fibronectin CS1 peptide (GeneArt) for the given timepoints. .. The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Pho

    Article Title: Oncofetal Chondroitin Sulfate Glycosaminoglycans are Key Players in Integrin Signaling and Tumor Cell Motility
    Article Snippet: The cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs.The cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs.. The cells were stimulated with 1-3% FBS, 5ug/ml FN (Sigma) or 10-80ug/ml fibronectin CS1 peptide (GeneArt) for the given timepoints.. The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Phosphatase and protease inhibitor cocktails (Roche).The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Phosphatase and protease inhibitor cocktails (Roche).

    Control:

    Article Title: Oncofetal Chondroitin Sulfate Glycosaminoglycans are Key Players in Integrin Signaling and Tumor Cell Motility
    Article Snippet: cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs. .. The cells were stimulated with 1-3% FBS, 5ug/ml FN (Sigma) or 10-80ug/ml fibronectin CS1 peptide (GeneArt) for the given timepoints. .. The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Pho

    Article Title: Oncofetal Chondroitin Sulfate Glycosaminoglycans are Key Players in Integrin Signaling and Tumor Cell Motility
    Article Snippet: The cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs.The cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs.. The cells were stimulated with 1-3% FBS, 5ug/ml FN (Sigma) or 10-80ug/ml fibronectin CS1 peptide (GeneArt) for the given timepoints.. The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Phosphatase and protease inhibitor cocktails (Roche).The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Phosphatase and protease inhibitor cocktails (Roche).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Oncofetal Chondroitin Sulfate Glycosaminoglycans are Key Players in Integrin Signaling and Tumor Cell Motility
    Article Snippet: cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs. .. The cells were stimulated with 1-3% FBS, 5ug/ml FN (Sigma) or 10-80ug/ml fibronectin CS1 peptide (GeneArt) for the given timepoints. .. The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Pho

    Article Title: Oncofetal Chondroitin Sulfate Glycosaminoglycans are Key Players in Integrin Signaling and Tumor Cell Motility
    Article Snippet: The cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs.The cells were washed in PBS and serum starved in the presence of 450nM rVAR2 or rDBL4 control for 18-24hrs.. The cells were stimulated with 1-3% FBS, 5ug/ml FN (Sigma) or 10-80ug/ml fibronectin CS1 peptide (GeneArt) for the given timepoints.. The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Phosphatase and protease inhibitor cocktails (Roche).The cells were put on ice, washed 3 times in PBS and lysed in EBC lysis buffer, containing 0.5% NP40 and Phosphatase and protease inhibitor cocktails (Roche).



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    Fig. 4. oFN acts through integrin a4 on osteoblasts. (A, B) Using an antibody that binds to integrin a4 called PS/2 prevents the inhibitory effect of oFN on mineralization, n ¼ 15/12/14/7 (A), and ERK phosphorylation, n ¼ 13/11/13/8 (B). (C, D) Administration of the peptide <t>CS1</t> containing the LDV sequence, which is a recognition site of a4b1 prevents the inhibitory effect of oFN on osteoblast differentiation, n ¼ 14/9/14/9 (C), and ERK phosphorylation, n ¼ 12/11/11/7 (D). (E) Administration of the FDC-6 antibody resulted in normalization of ERK phosphorylation, n ¼ 10/12/12/10. (F, G) Inhibiting a4b1 with a chemical compound (BIO5192, Tocris) diminishes osteoblast mineralization, n ¼ 12/7 (F), and ERK phosphorylation, n ¼ 8/11 (G) as achieved by oFN. p < 0.05, p < 0.01, p < 0.001.
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    Image Search Results


    Fig. 4. oFN acts through integrin a4 on osteoblasts. (A, B) Using an antibody that binds to integrin a4 called PS/2 prevents the inhibitory effect of oFN on mineralization, n ¼ 15/12/14/7 (A), and ERK phosphorylation, n ¼ 13/11/13/8 (B). (C, D) Administration of the peptide CS1 containing the LDV sequence, which is a recognition site of a4b1 prevents the inhibitory effect of oFN on osteoblast differentiation, n ¼ 14/9/14/9 (C), and ERK phosphorylation, n ¼ 12/11/11/7 (D). (E) Administration of the FDC-6 antibody resulted in normalization of ERK phosphorylation, n ¼ 10/12/12/10. (F, G) Inhibiting a4b1 with a chemical compound (BIO5192, Tocris) diminishes osteoblast mineralization, n ¼ 12/7 (F), and ERK phosphorylation, n ¼ 8/11 (G) as achieved by oFN. p < 0.05, p < 0.01, p < 0.001.

    Journal: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

    Article Title: An O-Glycosylation of Fibronectin Mediates Hepatic Osteodystrophy Through α4β1 Integrin.

    doi: 10.1002/jbmr.2916

    Figure Lengend Snippet: Fig. 4. oFN acts through integrin a4 on osteoblasts. (A, B) Using an antibody that binds to integrin a4 called PS/2 prevents the inhibitory effect of oFN on mineralization, n ¼ 15/12/14/7 (A), and ERK phosphorylation, n ¼ 13/11/13/8 (B). (C, D) Administration of the peptide CS1 containing the LDV sequence, which is a recognition site of a4b1 prevents the inhibitory effect of oFN on osteoblast differentiation, n ¼ 14/9/14/9 (C), and ERK phosphorylation, n ¼ 12/11/11/7 (D). (E) Administration of the FDC-6 antibody resulted in normalization of ERK phosphorylation, n ¼ 10/12/12/10. (F, G) Inhibiting a4b1 with a chemical compound (BIO5192, Tocris) diminishes osteoblast mineralization, n ¼ 12/7 (F), and ERK phosphorylation, n ¼ 8/11 (G) as achieved by oFN. p < 0.05, p < 0.01, p < 0.001.

    Article Snippet: For stimulation, the following substances were used: Human pFN and oFN isolated from outdated plasma or from amniotic fluid from midterm pregnancy using gelatin-sepharose-G4 gel were used at 25mg/mL. (13,20) FCS was depleted of fibronectin similarly as described and tested by ELISA. (10) CS1 peptide (DELPQLVTLPHPNLHGPEILDVPST) and the scrambled control (CT) peptide (GDPELNITLSVPLPTHLQEPDPVLH) (25mg/mL) were synthesized on an ABI 433 peptide synthesizer (Life Technologies, Carlsbad, CA, USA) using standard Fmoc-chemistry on Rink amide resin (Merck KGaA, Darmstadt, Germany).

    Techniques: Phospho-proteomics, Sequencing

    Fig. 6. Pharmacologic modification of oFN effects in vivo. (A, B) Injecting the PS/2 antibody (A) and the CS1 peptide (B) daily for 10 days (in parallel to oFN administration) led to an improvement of trabecular BMD, PS/2 groups: n ¼ 7/8/8/5, CS1 groups: n ¼ 8/8/6/5. (C, D) Mice with CCl4-induced experimental liver fibrosis show an increase in oFN in the circulation after 6 weeks (C) and a decrease in both total and trabecular BMD (D), n ¼ 12/17. (E) Circulating oFN levels correlate negatively with both total and trabecular BMD in all mice, n ¼ 29. (F, G) Both the PS/2 antibody (F) and the CS1 peptide (G) prevent bone loss in experimental liver fibrosis in mice, PS/2 groups: n ¼ 9/8/12/9, CS1 groups: n ¼ 10/10/11/8. Fibrosis was induced for 2 weeks with CCl4. After that, administration of CCl4 continued for 2 more weeks, but at the same time, the various treatments were injected daily. p < 0.05, p < 0.01, p < 0.001.

    Journal: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

    Article Title: An O-Glycosylation of Fibronectin Mediates Hepatic Osteodystrophy Through α4β1 Integrin.

    doi: 10.1002/jbmr.2916

    Figure Lengend Snippet: Fig. 6. Pharmacologic modification of oFN effects in vivo. (A, B) Injecting the PS/2 antibody (A) and the CS1 peptide (B) daily for 10 days (in parallel to oFN administration) led to an improvement of trabecular BMD, PS/2 groups: n ¼ 7/8/8/5, CS1 groups: n ¼ 8/8/6/5. (C, D) Mice with CCl4-induced experimental liver fibrosis show an increase in oFN in the circulation after 6 weeks (C) and a decrease in both total and trabecular BMD (D), n ¼ 12/17. (E) Circulating oFN levels correlate negatively with both total and trabecular BMD in all mice, n ¼ 29. (F, G) Both the PS/2 antibody (F) and the CS1 peptide (G) prevent bone loss in experimental liver fibrosis in mice, PS/2 groups: n ¼ 9/8/12/9, CS1 groups: n ¼ 10/10/11/8. Fibrosis was induced for 2 weeks with CCl4. After that, administration of CCl4 continued for 2 more weeks, but at the same time, the various treatments were injected daily. p < 0.05, p < 0.01, p < 0.001.

    Article Snippet: For stimulation, the following substances were used: Human pFN and oFN isolated from outdated plasma or from amniotic fluid from midterm pregnancy using gelatin-sepharose-G4 gel were used at 25mg/mL. (13,20) FCS was depleted of fibronectin similarly as described and tested by ELISA. (10) CS1 peptide (DELPQLVTLPHPNLHGPEILDVPST) and the scrambled control (CT) peptide (GDPELNITLSVPLPTHLQEPDPVLH) (25mg/mL) were synthesized on an ABI 433 peptide synthesizer (Life Technologies, Carlsbad, CA, USA) using standard Fmoc-chemistry on Rink amide resin (Merck KGaA, Darmstadt, Germany).

    Techniques: In Vivo, Injection

    Identification of fibronectin as an α4β7 binding component of CHO cell proteins. The CHO cell proteins were labeled with 125I. (A) Comparison of proteins detected by autoradiography to 25 μg of proteins stained with Coomassie blue. Five major bands present in the Coomassie-stained gel were also identified by autoradiography, as indicated. (B) Proteins displayed α4β7-dependent binding to RPMI88666 cells after radiolabeling. (C) RPMI8866 cells were used to capture and identify α4β7 components. 125I-labeled protein was captured on cells in the presence or absence of MAb 2B4, and then the cell-bound material was dissociated and analyzed. Input protein was compared to cell-bound material by SDS-PAGE and autoradiography. Shown are the original autoradiograph and a contrast-enhanced image. The cells retained a major band of approximately 260 kDa and a minor band of 55 kDa.

    Journal: Journal of Virology

    Article Title: Extracellular Matrix Proteins Mediate HIV-1 gp120 Interactions with α 4 β 7

    doi: 10.1128/JVI.01005-17

    Figure Lengend Snippet: Identification of fibronectin as an α4β7 binding component of CHO cell proteins. The CHO cell proteins were labeled with 125I. (A) Comparison of proteins detected by autoradiography to 25 μg of proteins stained with Coomassie blue. Five major bands present in the Coomassie-stained gel were also identified by autoradiography, as indicated. (B) Proteins displayed α4β7-dependent binding to RPMI88666 cells after radiolabeling. (C) RPMI8866 cells were used to capture and identify α4β7 components. 125I-labeled protein was captured on cells in the presence or absence of MAb 2B4, and then the cell-bound material was dissociated and analyzed. Input protein was compared to cell-bound material by SDS-PAGE and autoradiography. Shown are the original autoradiograph and a contrast-enhanced image. The cells retained a major band of approximately 260 kDa and a minor band of 55 kDa.

    Article Snippet: For fibronectin CS1 detection, mouse monoclonal IgM against human fibronectin CS1 peptide (Santa Cruz Biotechnology; clone P1F11) was diluted 1:200.

    Techniques: Binding Assay, Labeling, Autoradiography, Staining, Radioactivity, SDS Page

    Heparin inhibits fibronectin-mediated gp120-α4β7 binding. 1086.d7 gp120 was incubated for 2 h with CHO cell proteins. Heparin sulfate or chondroitin sulfate was added at the indicated concentrations 10 min prior to incubation with RPMI8866 cells. The results are the means and standard deviations of three replicates; the red line is a best-fit curve of the heparin inhibition data.

    Journal: Journal of Virology

    Article Title: Extracellular Matrix Proteins Mediate HIV-1 gp120 Interactions with α 4 β 7

    doi: 10.1128/JVI.01005-17

    Figure Lengend Snippet: Heparin inhibits fibronectin-mediated gp120-α4β7 binding. 1086.d7 gp120 was incubated for 2 h with CHO cell proteins. Heparin sulfate or chondroitin sulfate was added at the indicated concentrations 10 min prior to incubation with RPMI8866 cells. The results are the means and standard deviations of three replicates; the red line is a best-fit curve of the heparin inhibition data.

    Article Snippet: For fibronectin CS1 detection, mouse monoclonal IgM against human fibronectin CS1 peptide (Santa Cruz Biotechnology; clone P1F11) was diluted 1:200.

    Techniques: Binding Assay, Incubation, Inhibition

    The recombinant human fibronectin fragment retronectin mediates gp120-α4β7 binding. (A) Plasma fibronectin, cellular fibronectin, and retronectin were biotinylated and tested for binding to RPMI8866 cells. The results of a single representative experiment are shown. (B) Western blot analysis of plasma fibronectin (pFn), cellular fibronectin (cFn), and retronectin. Shown are two parallel blots developed with either polyclonal fibronectin antiserum or monoclonal antibody to the fibronectin CS1 peptide; 1 μg of protein was loaded per lane. (C) Retronectin mediates gp120 binding to RPMI8866 cells. Biotinylated retronectin (+biotin) produces a binding signal, and nonbiotinylated retronectin (−biotin) does not. Binding of wild-type MW959 and MW959 with the V2 loop deleted was measured with (hatched bars) and without (solid bars) 2-h preincubation with retronectin. The results are the means and standard deviations of 3 replicate measures.

    Journal: Journal of Virology

    Article Title: Extracellular Matrix Proteins Mediate HIV-1 gp120 Interactions with α 4 β 7

    doi: 10.1128/JVI.01005-17

    Figure Lengend Snippet: The recombinant human fibronectin fragment retronectin mediates gp120-α4β7 binding. (A) Plasma fibronectin, cellular fibronectin, and retronectin were biotinylated and tested for binding to RPMI8866 cells. The results of a single representative experiment are shown. (B) Western blot analysis of plasma fibronectin (pFn), cellular fibronectin (cFn), and retronectin. Shown are two parallel blots developed with either polyclonal fibronectin antiserum or monoclonal antibody to the fibronectin CS1 peptide; 1 μg of protein was loaded per lane. (C) Retronectin mediates gp120 binding to RPMI8866 cells. Biotinylated retronectin (+biotin) produces a binding signal, and nonbiotinylated retronectin (−biotin) does not. Binding of wild-type MW959 and MW959 with the V2 loop deleted was measured with (hatched bars) and without (solid bars) 2-h preincubation with retronectin. The results are the means and standard deviations of 3 replicate measures.

    Article Snippet: For fibronectin CS1 detection, mouse monoclonal IgM against human fibronectin CS1 peptide (Santa Cruz Biotechnology; clone P1F11) was diluted 1:200.

    Techniques: Recombinant, Binding Assay, Western Blot